KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I
NCBI Gene#: 1452/
Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.
Human Mouse Rat
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at ‘Ser-45’. May play a role in segregating chromosomes during mitosis.Tapia C., FEBS Lett. 349:307-312(1994).Fish K.J., J. Biol. Chem. 270:14875-14883(1995).Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.