DNA2 Antibody, HRP conjugated

$210.00$377.00

Description

Aliases

DNA replication ATP-dependent helicase/nuclease DNA2, hDNA2, DNA replication ATP-dependent helicase-like homolog, DNA2, DNA2L, KIAA0083

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

P51530

Immunogen

Recombinant human DNA replication ATP-dependent helicase/nuclease DNA2 protein (1-300AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5′-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5′-ssDNA, while the 3′-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5′-3′ helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5′-3′ DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Isotype

IgG

Conjugate

HRP

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

>95%,Protein G purified

Additional information

Size

50μg, 100μg

Certificate of Analysis