Description
Aliases
ELK1; ETS-domain protein Elk-1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P19419
NCBI Protein: NP_001107595.
Immunogen
Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 47kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.Xing J, et al. (1996) Science. 273(5277): 959-963.Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.Marais R, et al. (1993) Cell 73: 381-393.Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser383
Additional information
| Size | 100?l |
|---|
