Goat anti-Mouse IgG, Horseradish peroxidase-HRP labeled


Goat Anti-Mouse IgG (H+L) Antibody HRP conjugated, purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads is useful in Western Blot, ELISA, IHC, and ICC protein identification techniques.


Product name: Goat Anti-Mouse IgG H&L HRP Labeled

Host species: Goat

Target species: Mouse

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Conjugation: HRP

Form: Liquid

Storage instructions: Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze and thawing.

Storage buffer: 50% Glycerol, 0.01M PBS, pH 7.4

Concentration: It differs from different batches, generally 0.1 mg/mL.

Purity: Immunogen affinity purified: isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Conjugation notes: Molar enzyme/ antibody protein ratio is 4:1

Clonality: Polyclonal

Isotype: IgG

Tested applications: Western Blot, ELISA, ICC, IHC-P

Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:



Recommended Dilution

Western Blot

1:500 – 1:1000
Chemiluminescent detection

1:3000 – 1:5000
Colorimetric detection


1:500 – 1:10000


Fig: Western blot – Probed with Goat Anti-Mouse IgG H&L (HRP) 
All lanes: Anti-beta Actin antibody, Loading Control at 1 µg/ml
Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1 – 2: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:2000 dilution
Lanes 3 – 4: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:10000 dilution
Lanes 5 – 6: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:20000 dilution
Developed using the ECL technique
Exposure time: 10 seconds

Additional information


0.5 mL, 1.0 mL

Certificate of Analysis