ROD; AUF1; HNRPD;
NCBI Gene#: 3184;
Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD .
WB IHC IF Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3′-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5′-UUAG-3′ sequence and also weaker to the single-stranded 5′-TTAGGG-3′ telomeric DNA repeat. Binds RNA oligonucleotides with 5′-UUAGGG-3′ repeats more tightly than the telomeric single-stranded DNA 5′-TTAGGG-3′ repeats. Kajita Y., J. Biol. Chem. 270:22167-22175(1995).Dempsey L.A., Genomics 49:378-384(1998).Tolnay M., Biochem. J. 338:417-425(1999).Grosset C., Cell 103:29-40(2000)
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.