HSF1(Phospho-Ser307) Antibody

$454.00

Cat. No.: 39015 Categories: ,

Description

Aliases

HSTF 1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q00613
NCBI Protein: NP_005517.1

Immunogen

Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IF Recommended dilution: Predicted MW: 82kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4 Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026 Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser307

Additional information

Size

100?l

Certificate of Analysis