Description
Aliases
AP-1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P17275
NCBI Protein: NP_002220.1
Immunogen
Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 43kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5′-TGA[CG]TCA-3′.Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser259
Additional information
Size | 100?l |
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