Description
Aliases
HDMX, hdm2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q00987
NCBI Protein: NP_002383.2
Immunogen
Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 90kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissuesHaupt, Y. et al. (1997) Nature 387, 296-299.Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser166
Additional information
| Size | 100?l |
|---|
