MOPS-SDS Running Buffer [20X]

MOPS-SDS Running Buffer [20X] (1M MOPS, 1M Tris, 20.5mM EDTA, 69.3mM SDS)

MOPS-SDS Running Buffer is specially formulated to be used for running proteins on a gel. This running buffer recommended for separating small- to medium-sized proteins, provides increased resolution of small MW proteins, and shorten the electrophoresis run time. MOPS buffer allows proteins to run slower than when using MES buffer.

MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.

MOPS SDS Running Buffer (20X) is recommended for separating medium- to large-sized proteins. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

All of our buffers are made with highest purity reagents with strict quality control and are provided as a concentrated solution, which simply require dilution with de-ionized water/buffer to use. This running buffer needs to be supplemented with 1X working concentration of an antioxidant to prevent protein re-oxidation during electrophoresis when running reduced protein samples.