MOPS, also known as 4-Morpholinepropanesulfonic acid and 3-(N-Morpholino) propane sulfonic acid is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS has a buffering pH range of 6.5-7.9. It is widely used in biological and biochemical studies due to its inert properties and does not interact with any metal ions in solutions. It displays significant metal-buffer stability especially with copper, nickel, manganese, zinc and cobalt ions. MOPS buffer maintains the pH of the mammalian cell culture medium and in denaturing gel electrophoresis of RNA. It can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS is known to interact with bovine serum albumin to stabilize the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.
InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N
pH: 2.5-4 (25°C, 209 g/L)
pKa (25 °C): 7.2
λ: 33 % in H2O
- a cell culture additive component in lentiviral particle production
- as a buffering agent in microbial growth medium and nuclei extraction buffer
100 g, 250 g