Nuclear Fast Red Staining Solution is a common nuclear counterstain formulation composed of an anionic anthraquinone dye, used in conjunction with a mordant, usually an aluminum salt. Also called as Kernechtrot or Calcium Red, Nuclear Fast Red Stain is a highly versatile reagent that selectively stains nuclear chromatin red, while the nonspecific signal is detected in pink tones. This staining solution offers a faster, optimized, intense and simpler multi-labeling nuclear counterstain alternative in comparison to hematoxylin, in the cases where hematoxylin would normally conceal the substrate signal resulting in poor resolution.
Nuclei – red
Cytoplasm – pale pink
Note: Precipitation might occur with Nuclear Fast Red Stain Solution which is easily resuspended in the solution upon gentle shaking prior to each use, DO NOT FILTER!
Features:
• Saves time – rapid and simple staining protocol
• Good resolution – high contrast with various substrates
• Compatible with non-aqueous mounting reagents
Applications:
• Immunohistochemistry and immunocytochemistry-based assays
• In situ hybridization
Nuclear Fast Red Chemical Details:
Molecular Weight: 357.27 g/mol
Molecular Formula: C14H8NNaO7S
Synonyms: Kernechtrot; Calcium Red; Sodium 1-amino-2,4-dihydroxy-9,10-dihydro-9,10-dioxoanthracene-3-sulphonate; CI 60760; Helio Fast Rubin BBL; 2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt
CAS No.: 6409-77-4
MDL No.: MFCD00078493
EC No.: 229-088-0
InChI Key: IFSXZLJQEKGQAF-UHFFFAOYSA-M
PRODUCT CITATION:Â
Kim, Hee Eun et al (2024) Amplifying endogenous stem cell migration for in situ bone tissue formation: Substance P analog and BMP mimetic peptide-loaded click-crosslinked hyaluronic acid hydrogel. MATERIALS TODAY BIO https://doi.org/10.1016/j.mtbio.2024.101070
