PLD2 (Phospho-Tyr169) Antibody

$454.00

Cat. No.: 38932 Categories: ,

Description

Aliases

PLD 2; PLD1C; choline phosphatase 2;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: O14939;
NCBI Gene#: 5338;
N

Immunogen

Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.Steed P.M., FASEB J. 12:1309-1317(1998).Lopez I., J. Biol. Chem. 273:12846-12852(1998).Divecha N., EMBO J. 19:5440-5449(2000).

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr169

Additional information

Size

100?l

Certificate of Analysis