Ponceau S Dye Powder

Description

Ponceau S is the sodium salt of a diazo dye, used to make Ponceau S Stain Solution for rapid reversible staining of protein bands on Western Blots made with Nitrocellulose or PVDF membranes. The Ponceau S Stain Solution is also used for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The most common formulation of Ponceau S Stain Solution contains 0.1% Ponceau S (w/v) in 5% Acetic acid.

Chemical Name: 3-Hydroxy-4[2-sulfo-4-(4-sulfophenylazo) phenylazo]-2,7-naphthalenedisulfonic acid

Molecular Formula: C₂₂H₁₂N₄Na₄O₁₃S₄

Molecular Weight: 760.60

CAS NO: 6226-79-5

Purity Limit: ≥ 85% (Dye content

Solubility:  Readily soluble in water to give a clear red solution

UV Ratios: Absorptivity 1%, 1 cm: λmax ~520 nm

Loss on Drying: ≤ 5.0%

Appearance: Red-brown to dark-brown powder

Additional information