SAPK/JNK(Phospho-Thr183) Antibody

$454.00

Cat. No.: 39024 Categories: ,

Description

Aliases

JNK2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P45984/P53779
NCBI Protein: NP_00

Immunogen

Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 46 54 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23 Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206 D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr183

Additional information

Size

100?l

Certificate of Analysis