Our Tris-Acetate-SDS Running Buffer [20X] contains 1 M Tris, 1 M Tricine and 2 % SDS. When diluted to 1X with DI Water, it gives 50 mM Tris, 50 mM Tricine, 0.1 % SDS and pH ~ 8.24.
This buffer is formulated for running proteins on Bis-Tris and Tris-Acetate gels, including ThermoFisher Scientific NuPAGE® gels, and is preferred for separating small- to medium-sized proteins. Tris-Acetate-SDS Running Buffer when used with Tris-Acetate gels, provide an excellent separation of large molecular weight proteins. This buffer is formulated for separation of proteins in their denatured state to resolve native proteins more effectively.
500 ml, 1L