High affinity nerve growth factor receptor precursor; NTRK1; Slow nerve growth factor receptor; TRK; TRK1 transforming tyrosine kinase protein
NCBI Protein: NP_001007793.
Peptide sequence around phosphorylation site of tyrosine791 (P-V-Y(p)-L-D) derived from Human TrkA.
WB IF Recommended dilution: Predicted MW: 140kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.Wiese S, et al. Proc Natl Acad Sci U S A. 2007 Oct 23; 104(43):17210-5.Valdez G, et al. Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12270-5Inoue K, et al. J Biol Chem. 2007 Aug 17;282(33):24175-84
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.