Biotin-PEG3-Amine

General Description

Biotin-PEG3-Amine is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinking procedures. The short PEG segment in this compound is hydrophilic and confer greater solubility to labeled proteins. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy terminal, aspartate or glutamate residues using EDC, DCC or activated esters e.g. NHS esters. EDC activates carboxyl groups to bind to the -NH2 group of the
amino-biotin, forming an amide bond.

Chemical Name:             (+)-Biotinyl-3,6,9-trioxa dioxaundecaneediamine

Molecular Formula:        C₁₈H₃₄N₄O₅S

Molecular Weight:          418.55

CAS NO.:                           359860-27-8

Purity:                               98%

Spacer arm length:         22.9A⁰

Form:                                 White to off-white solid

Solubility:                         ~25mg/ml in water

Storage:                            4⁰C

Features of Biotin-PEG3-Amine:

• Biotinylation— label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins

• Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers

• Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules

• Medium length—spacer arm (total length added to target) is 22.9 Å