Cat. No.: 10532 Categories: ,


General Description

Biotin-PEG3-Amine is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinking procedures. The short PEG segment in this compound is hydrophilic and confer greater solubility to labeled proteins. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy terminal, aspartate or glutamate residues using EDC, DCC or activated esters e.g. NHS esters. EDC activates carboxyl groups to bind to the -NH2 group of the
amino-biotin, forming an amide bond.

Chemical Name:             (+)-Biotinyl-3,6,9-trioxa dioxaundecaneediamine

Molecular Formula:        C18H34N4O5S

Molecular Weight:          418.55

CAS NO.:                           359860-27-8

Purity:                               98%

Spacer arm length:         22.9A0

Form:                                 White to off-white solid

Solubility:                         ~25mg/ml in water

Storage:                            40C


Features of Biotin-PEG3-Amine:

Biotinylation— label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins

Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers

Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules

Medium length—spacer arm (total length added to target) is 22.9 Å


Additional information


50 mg

Certificate of Analysis