Description
Aliases
CDC25M3; MPIP1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P30304
NCBI Protein: NP_001780.2
Immunogen
Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.Jin J, et al. (2003) Genes 17(24): 3062-74.Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser76
Additional information
Size | 100?l |
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