Caspase-10 Assay Kit, Fluorometric with Ac-AEVD-AFC substrate

$305.00$471.00

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Cat. No.: 10083 Categories: ,

Description

Caspase-10 Assay Kit, Fluorometric with Ac-AEVD-AFC substrate

 

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by Caspase-10 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.

 

Detection Method:         Fluorescence (Ex/Em 400 nm/505 nm)

Species Reactivity:         Mammalian

Applications:                   Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis

Storage temp.:                -20°C

 

Features & Benefits:

  • Simple one-step procedure that takes only ~1-2 hours
  • Fast and convenient
  • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent caspase activity

 

Kit Components:

  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • AEVD-AFC (1 mM)
  • DTT (1 M)

Additional information

Size

50 Assays, 100 Assays