FADD (Phospho-Ser191) Antibody

$454.00

Cat. No.: 38934 Categories: ,

Description

Aliases

MORT1 ; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: Q13158;
NCBI Gene#: 14082;

Immunogen

Peptide sequence around phosphorylation site of Serine191(R-S-G(p)-A-M) derived from Human FADD.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.Sugano S., Nat. Genet. 36:40-45(2004).Farmer A., Submitted (MAY-2003).Venter J.C., Submitted (JUL-2005).

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser191

Additional information

Size

100?l

Certificate of Analysis