FBXL3 (Phospho-Ser320) Antibody

$454.00

Cat. No.: 38722 Categories: ,

Description

Aliases

FBL3A; FBXL3A

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: Q9UKT7
NCBI Protein#: NP_036290.1

Immunogen

Peptide sequence around phosphorylation site of serine 320 (S-V-S(p)-K-D) derived from Human FBXL3.

Raised In

Rabbit

Species Reactivity

Humann Mouse Rat

Tested Applications

WB Recommended dilution: Western blotting: 1:500~1:1000

Background / Function

Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2. Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex.1)Busino L., Bassermann F., Maiolica A., Lee C., Nolan P.M., Godinho S.I., Draetta G.F., Pagano M. Science 316:900-904(2007) 2)Yoo S.H., Mohawk J.A., Siepka S.M., Shan Y., Huh S.K., Hong H.K., Kornblum I., Kumar V., Koike N., Xu M., Nussbaum J., Liu X., Chen Z., Chen Z.J., Green C.B., Takahashi J.S.Cell 152:1091-1105(2013)

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser320

Additional information

Size

100?l

Certificate of Analysis