FOS (Phospho-Thr232) Antibody

$454.00

Cat. No.: 38918 Categories: ,

Description

Aliases

FOS; G0S7; Cellular oncogene fos;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: P01100;
NCBI Gene#: 2353;
N

Immunogen

Peptide sequence around phosphorylation site of threonine 232(V-A-T(p)-P-E) derived from Human FOS .

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Background / Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983).Hai T., Genes Dev. 3:2083-2090(1989).Heilig R., Nature 421:601-607(2003).

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr232

Additional information

Size

100?l

Certificate of Analysis