Growth factor receptor bound protein 2-associated protein 1
NCBI Protein: NP_002030.2
Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.
WB IF Recommended dilution: Predicted MW: 110kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 – 17904.Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.