Geneticin, also known as G418, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. It causes a chain of nonsense mutations during translation, resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to Geneticin is conferred by the neo gene encoding an aminoglycoside 3′-phosphotransferase, APH 3′ II. Geneticin is commonly used in laboratory research to select genetically engineered cells.
CAS Number: 49863-47-0
Synonyms: Antibiotic G 418; G418; Antibiotic G-418; BRN 1669188
Molecular Formula: C20H40N4O10.2H2SO4
Molecular Weight: 692.71
InChI Key: BRZYSWJRSDMWLG-DJWUNRQOSA-N
1 g, 5 g