PBST (phosphate buffered saline with Tween20) is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The solution consists of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. Under a proper buffering environment, the unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
The PBST Wash Buffer is Phosphate Buffered Saline with Tween and supplied at 10X concentration with 0.05% Tween 20.
The chemicals used in the manufacturing of PBST Wash Buffer are of highest purity and prepared under GMP guidelines.
250 ml, 500 ml