Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands. Tris-glycine-SDS running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both anode and cathode buffer. This buffer is also used to block membranes with 5% non-fat dry milk for western blotting.
Our Tris-Glycine-SDS Running Buffer is used in running SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is supplied as 10X concentration and can be quickly diluted to use in SDS-PAGE. When diluted to 1X in water, the solution yields 0.025M Tris, 0.192M Glycine and 0.1% SDS (Sodium dodecyl sulfate) with pH 8.3-8.5.
Features of Tris-Glycine-SDS Buffer [10X]:
• Supplied as 10X concentration, saves time in making and space saver
• Ready-to-use, no chemicals to weigh, dissolve and adjust the pH
• Increased accuracy— eliminates the possibility of errors in weighing and transferring the chemicals
500 ml, 1 L, 1 Gal