Protease Assay Kit

Cepham Life Sciences, protease assay kit is a sensitive, universal protease assay kit, which is designed for the quantitative determination of the proteases present in the protein sample and can detect approximately 10ng/ml of proteases in 2 to 24 hours incubation. The kit is supplied with a protease substrate: casein labeled with resorufin and with protease activities in the test sample, resorufin-labeled peptides are released, that cannot be precipitated by the precipitating agent. The concentration of these resorufin-labeled peptides in the supernatant is directly proportional to the proteolytic activity present in the test sample, which can be measured spectrophotometrically at 574nm.

Proteases are enzymes, naturally present in all living cells and tissues, involved in a multitude of physiological reactions that includes digestions of proteins in the food to highly regulated cascades. Proteases can either break specific peptide bonds, depending on the amino acid sequence of a protein or completely breakdown peptides to amino acids. In certain physiological conditions, protease activity can abolish a protein’s function or an activation of a function. For studying proteins, tissues and cells are lysed, which release proteases in the sample and their activities can be measured using a protease assay.

Our Protease Assay kit has been optimized for the assay of general proteases present in a sample, utilizing resorufin labeled casein as the assay substrate. It is a highly sensitive kit and precisely measures the protease activity in the biological or any other sample possessing protease activity.