Reassembly Buffer (RAB)

Description

General Description

A high salt, reassembly buffer for lysis.

Our Reassembly Buffer (RAB) is used in the sequential extraction of proteins and contains high salt concentration (750 mM NaCl), 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), 1 Ethylene glycoll bis-2-aminoethylether-N,N′,N′′,n′-tetraacetic acid (EGTA), 0.5 mM Magnesium sulfate (MgSO4), 20 mM Sodium fluoride (NaF) at pH 7.0.

RAB has commonly been used for extracting a microtubule-associated protein- Tau1 and soluble proteins from different cell lines such as HEK2, other cells and tissues.

References:

1. Eckermann, K., et al, J. Biol Chem, 2007, 282(43), 31755-31765

2. Chun, W., et al, J Neurochemistry, 2007, 103, 2529-2539

Additional information