Description
Aliases
SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P29353
NCBI Protein: NP_001123512.
Immunogen
Peptide sequence around phosphorylation site of tyrosine 349 (H-Q-Y(p)-Y-N) derived from Human Shc1.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IF Recommended dilution: Predicted MW: 46 52 kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044.Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6(11): 1435-1444
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr349
Additional information
Size | 100?l |
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