Description
Aliases
CAV; CAV1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q03135
NCBI Protein: NP_001166366.
Immunogen
Peptide sequence around phosphorylation site of tyrosine 14 (H-L-Y(p)-T-V) derived from Human CAVEOLIN-1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IF Recommended dilution: Predicted MW: 24kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250.Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140.Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586.Labrecque L, et al. (2003) Mol Biol Cell. 14(1): 334-347.Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr14
Additional information
| Size | 100?l |
|---|
