Description
Aliases
kinase Cdk2; p33 protein kinase;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P24941
NCBI Protein: NP_001789.2
Immunogen
Peptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from Human CDK2.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 34kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr160
Additional information
Size | 100?l |
---|