Description
Aliases
CFL1/CFL2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P23528 Q9Y281
NCBI Protein: NP _00
Immunogen
Peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y(p)-E-T) derived from Human coflin1/cofilin2.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
IHC WB Recommended dilution: Predicted MW: 19kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.Arber, S. et al. (1998) Nature 393, 805-809.Yang, N. et al. (1998) Nature 393, 809-812.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr88
Additional information
| Size | 100?l |
|---|
