CMT2F, HMN2B, HSP27, HSP28, Hsp25
NCBI Protein: NP_001531.1
Peptide sequence around phosphorylation site of serine15 (G-P-S(p)-W-D) derived from Human HSP27.
WB IHC IF Recommended dilution: Predicted MW: 27kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Involved in stress resistance and actin organization. Tremolada L, et al. (2005) Proteomics. 5(3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124. Benn SC, et al. (2002) Neuron. 36(1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.