General Description
NHS-Biotin reacts with primary amines to biotinylate proteins under mild conditions. It has been used to prepare a biotinylated cytidine as a probe in the detection of related DNA sequences, and for biotinylating myeloma cells and immunogens to form a bridge with streptavidin to improve relevant hybridoma formation.
Name:Â Â Â Â Â Â Â Â Â Â Â Â Â Â N-Hydroxysuccinimido Biotin
Synonyms:Â Â Â Â Â Â Â Â Â Â NHS Biotin; 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (+)Biotin N-hydroxysuccinimideester
CAS Number:Â Â Â Â Â Â Â Â 35013-72-0
Molecular Weight:Â Â Â Â 341.38
Color & Form:Â Â Â Â Â Â Â Â White fine-grained powder
Melting Point:        210-212° C
Molecular Formula:Â Â Â C14H19N3O5S
Spacer Arm:Â Â Â Â Â Â Â Â Â 13.5 Ã…
Reactive group(s):Â Â Â Â N-hydroxysuccinimide ester reacts with primary amines at pH 7.0-9.0
