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Coomassie Brilliant Blue G-250 Dye

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Description

General description

Coomassie Brilliant Blue G-250 is one of the most commonly available and utilized form of coomassie dye, which is a key component of various colorimetric protein gel stains. It is a chemical form of a disulfonated triphenylmethane compound that is commonly employed as the basis of stains for protein detection and quantitation in gel electrophoresis and Bradford-type assay reagents. G-250 (green-tinted) form is also referred to as colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

 

CAS#:                                6104-58-1

Molecular Formula:        C47H48N3O7S2Na

Molecular Weight:           854

Appearance:                     Dark blue to Blue-black to Dark brown to Violet-brown powder

Solubility:                          Dark blue solution (1 mg/mL in water)

Storage temp.:                 Store at RT

Suitability:                        Suitable for electrophoresis

 

Applications/ Features:

  • Easy detection; develops intensely colored complexes with proteins
  • High sensitivity; can determine as little as 0.5 µg/cm2 of protein present in a gel matrix
  • Reversible staining; anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions
  • Differentiation between bound/unbound dye; when dissolved in 0.01M citrate buffer at pH 3.0, has an absorption maximum at 555nm; protein-dye complex is characterized by a peak slightly broader than that of the free dye with a maximum at 549 nm

 

Additional information

Size

25 g, 50 g, 100 g, 250 g, 500 g, 1 Kg

Certificate of Analysis