PVDF is a hydrophobic membrane used for a wide variety of blot, sequencing, and protein analysis protocols. Due to the hydrophobic character of the membrane, there is a much higher rate of protein retention. It is supplied pre-cut in 8.5cm x 7.5cm and 10cm x 10cm sizes with blotting paper to make it ready to use.
The supplied PVDF Membrane pore size is 0.2 µm, has excellent binding properties and demonstrate a high level of compatibility with different detection methods, such as chemiluminescent and colorimetric western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing. These membranes retain target protein very strongly during the protein transfer but also reduce the nonspecific protein binding that can obscure high-sensitivity detection. The 0.2-µm pore size makes these membranes ideal for protein analysis of small amounts of proteins (as little as 10 pM), amino acid analysis, and transfer of small molecular weight proteins and peptides.
- Membrane: polyvinylidene difluoride (PVDF)
- Re-probe characteristics: Mechanical strength of PVDF membrane enables multiple reprobing of blots
- Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
- Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
- Durability: compatible with most organic solvents, acids, and mild bases; Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
- Transferring proteins from SDS-PAGE gels to membrane
- Immunoblotting target proteins
- Detection by chemiluminescent or colorimetric western blotting methods
8.5 cm x 7.5 cm, 10 cm x 10 cm