PTN11; PTP-1D; PTP-2C; PTP2C; PTPN11
NCBI Protein: NP_002825.3
Peptide sequence around phosphorylation site of tyrosine 580 (R-V-Y(p)-E-N) derived from Human SHP-2.
Human Mouse Rat
WB IHC Recommended dilution: Predicted MW: 72kd, Immunohistochemistry: 1:50~1:100
Background / Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Ferjoux G, et al. (2003) Mol Biol Cell. 2003 ; 14(9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682 Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.