Description
General description
MBS and its water-soluble analog Sulfo-MBS are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups, allowing for covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction where the rate increases with increasing pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. Hence, conjugation experiments employing these crosslinkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneously with the maleimide (sulfhydryl-targeted) reaction.
InChI Key: LKUULGDICDGFIQ-UHFFFAOYSA-M
Form: Powder
Molecular Weight: 416.3
Storage cond.: Desiccated
Solubility: Water soluble
Storage temp.: −20°C
Features and Benefits:
- Reactive groups are Sulfo-NHS ester and maleimide
- Reactive towards amino and sulfhydryl groups
- Non-cleavable
- Water-soluble (compare to MBS)
- Membrane impermeable, allowing cell surface labeling
Additional information
| Size | 100 mg |
|---|
