Cat. No.: 10529 Categories: ,


General Description

The compound Biotin LC Hydrazide is a mid-length hydrazide-activated biotinylation reagent for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes. It is highly soluble in DMSO and membrane permeable. It enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of antibodies with sodium periodate produces reactive aldehydes on the carbohydrate moieties of the Fc portion that can be modified by hydrazides. This approach is advantageous for labeling antibodies since biotinylation occurs only at the sites of glycosylation, which are primarily in the Fc region of the antibody, far from the antigen binding site.


Chemical Name:         6-(biotinamido)hexanehydrazide

Molecular Formula:    C16H29N5SO3

Molecular Weight:      371.50

Spacer arm length:     24.7 Å

CAS No.:                       109276-34-8

Form:                             White to off-white powder

Solubility:                      Highly soluble in DMSO (50mM), less soluble in water (5mM) and poorly soluble in DMF

Reactive Group:           Hydrazide reacts with aldehydes at pH 4to 6


Features and Benefits:

·        Glycoprotein labeling— biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins

·        Cell surface labeling— biotinylate and isolate cell surface glycoproteins

·        Aldehyde-reactive— reacts with aldehydes formed by periodate-oxidation of sugar groups

·        Hydrazide-activated— perform reactions at pH 4 to 6 in buffers such as sodium acetate

·        Irreversible— forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved

·        Solubility— soluble in DMSO, then further dilution in aqueous buffers

·        Spacer arm length—24.7Å


Additional information


50 mg

Certificate of Analysis