Our SDS-PAGE Sample Loading Buffer is based on Bromophenol blue dye, supplied as 6X concentration and contains Tris buffer, SDS, Glycerol and Bromophenol blue (BPB) dye at pH 6.8, based on Laemmli Buffer. It is 0.22um filtered ready to use solution, simply mix one-part Sample Buffer with five-parts protein sample, add recommended concentration of an appropriate reducing agent (e.g. 20 mM DTT or 5% BME), heat denature and load on the gel
Features of SDS-PAGE Sample Loading Buffer [6X]:
• Ready to use— Add directly to the sample with a reducing agent and load on the gel
• 6X Concentrated— Enables a larger volume of protein solution to be included in the sample that is loaded in each well, being 6X concentrated.
1. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227. 680-685. PMID: 5432063
5 ml, 25 ml