Our Taq DNA polymerase with Buffers and dNTPs (PCR Mastermix) is a ready-to-use reaction mixture, which only requires the researcher to add molecular grade water, DNA template and 5’ and 3′ primers.
The supplied Taq DNA Polymerase in the Mastermix is originated from Thermus Aquaticus with a molecular weight of 94KDa.
This Mastermix can be used in the DNA fragment extension amplification reactions with DNA having dA overhang on 3’ ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75ºC. The Mastermix has been optimized for the best performance, when used with dNTP at 100-300 mM, 1.5-3.0 mM Mg2+ concentrations, and pH between 8.1-9.2.
500 U, 1000 U