The Nickel-NTA or Ni-NTA Agarose Resin consists of beads of divalent nickel ions (Ni2+) and highly cross-linked 6% Agarose, coupled with Nitilotriacetic acid (NTA, a tetradenate chelating ligand). Ni-NTA Agarose resin is is a high-capacity, high-performance nickel-IMAC resin specifically designed for affinity purification of His-tagged (6XHis) fusion proteins. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. The Ni-NTA Resin can be used to purify 6x His-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.
NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni2+ and is ideal for rapid purifications of His-tagged proteins. Ni-NTA resins are a common choice for IMAC-based 6xHis-tag protein purifications due to the availability of four metal-binding sites on the chelate, enabling high-protein binding and low-metal ion leaching. The purity and yield of the target protein depends on working conditions, size, conformation, expression levels, solubility and sample impurities.The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives and can be used under native and denaturing conditions to recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells.
- Convenient one step purification of His-tagged proteins; high performance
- High-protein binding and low-metal ion leaching
- Protein purification compatible with native or denaturing conditions
- Recover His-tagged proteins expressed in baculovirus, yeast, mammalian and bacterial cells
- More specificity than Ni-IDA Agarose resin
10ml, 25 ml, 100 ml